Vegetative Propagation of Coleus forskohlii (Wild) Briq using their Stem Cutting for Ex-situ Conservation in Herbal Garden
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چکیده
Biodiversity includes variation and variability among the different living species of different natural habitats. Out of them plant diversity also placed in significant construction of biodiversity. Plant diversity is affected by several biotic and abiotic factors and is also regulated by their genes. Plant life is always affected by their population structure, natural effect in different phases of their life.
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Coleus spp.: Micropropagation and In Vitro Production of Secondary Metabolites
Coleus spp. have long been prized for their colorful foliage, which may combine shades of green, yellow, pink, red and maroon. They are plants for all locations. Besides their ornamental nature, Coleus spp. are also valued in folk medicine. C. blumei Benth., a natural hybrid of several Coleus spp. is an ornamental plant growing all over the world in an enormous number of different cultivars tha...
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MS liquid medium supplemented with 3 mg/l NAA and 1 mg/l BAP with pH 5.7 was used for suspension cultures. Suspension cultures subjected to biotic elicitors Aspergillus niger, Fusarium oxysporium, Rhizopus oryzae and Penicillium notatum induced increase in forskolin content. Among all the elicitors tried Aspergillus niger proved to be best elicitor at 5% CF which was 6 times higher than the con...
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The aim of this study was to elucidate the effect of elicitors and precursors on the production of forskolin from the hairy root cultures of Coleus forskohlii Briq. Hairy root cultures were established from leaf explants by infecting with Agrobacterium rhizogenes strain A4 on MS basal medium. Suspension cultures of hairy root cultures were initiated in MS medium containing IBA (1.0 mg/L), casei...
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A rapid method was developed for the evaluation of forskolin in Coleus forskohlii Briq. (Lamiaceae). Forskolin was quantitated in the root and stem of dried C. forskohlii and in 17 market products by reversed-phase liquid chromatography (LC) with a photodiode array detector at 210 nm. The temperature was held constant at 30 degrees C, and the retention time of forskolin was approximately 6.8 mi...
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